Embryonic and Ovocitary Vitrification
The greatest innovation in the world of assisted reproduction, since the introduction of intracytoplasmic sperm microinjection (ICSI) is undoubtedly ovocitary / embryonic vitrification.
This technique offers really amazing success and survival rates in terms of post defrosting survival, great embryonic development capacity (cell division) during in vitro culture. The scientific innovation of this exclusive CEFAM technique is based on the ultra-fast cooling of cells, through interaction with protective agents in storage conditions under a minimum volume of medium which is based on the Japanese technique implemented by Doctor Kuwayama .
Traditionally, the cells, being composed of water in more than 85% of their volume, when raised were preserved by means of the "slow" freezing protocol, when interacting with the protective agents, ice crystals formed within the ultra structures that make up the cell caused by the decrease in temperature so slow, which acted as authentic blades that caused permanent damage to the integrity of the cell offering poor results in embryonic defrosting (and even lower in the case of defrosting of ovules).
Through vitrification and interaction with protective agents in conditions of minimum volume and cooling rates close to - 23,000 degrees per minute, deep freezing maintains integrity in the cellular ultra structure since they are not able to form ice crystals, but a “vitreous” substance is formed causing the least damage, allowing survival and pregnancy rates in the transferred embryos derived from this technique really amazing and in some cases even superior to a cycle performed “in fresh” by a treatment of assisted reproduction of high complexity.